Annotated Phosphosites-Kinase

Product Code: KIN-MA-PhK

Collection of 720 annotated human phosphorylation site derived peptides (13mers, sequences are identical to Kinase Substrate Set).Amount:1 peptide microarray (each peptide is deposited 3x per subarray, 3 subarrays per slide) + dummy slide and spacers (needed for manual incubation)Delivery Format: Peptides immobilized onto glass slide (3 x 1 inches; 75 by 25 mm)Application(s): Phosphosite detection, Substrate identificationIndication(s)/Topic(s): KinaseDelivery Time: 10-12 days

Kinase Substrate Microarrays JPT Peptide Technologies applies its unique peptide microarray platform to generate peptide microarrays on glass slides enabling characterization of enzymes such as kinases as well as methyl, glycosyl, acetyl transferases. Peptides are immobilized on glass slides via a flexible linker. Chemoselective coupling generates microarrays displaying covalently attached peptides in four identical subarrays (3 copies of peptide within each subarray). Incubation can be performed manually or automated.

Benefits of JPTs Kinase Substrate Microarrays - Get hundreds of identical microarray copies - Peptides are free of truncated sequences - Small amounts of your enzyme preparation is needed for incubation - Applicable to a variety of biological samples (e.g. serum, lysate, kinase) - Low background on glass surface - Read-out using fluorescenctly labeled antibodies or radioisotopically labeled ATP - Screen thousands of potential peptide substrates economically

Annotated Phosphosites-Kinase

Selected References \"Phosphopeptide Detection with Biotin-Labeled Phos-tag\" Kinoshita-Kikuta et al., Methods in molecular biology (2016) - PMID: 26584916 \"Phosphotyrosine Substrate Sequence Motifs for Dual Specificity Phosphatases\"Zhao et al., PLoS One. (2015) - PMID:26302245 \"Phos-tag Technology for Kinomics\"Kinoshita-Kikuta et al., Kinomics: Approaches and Applications (2015) - PMID: n.a. \"Characterization of the Host Response to Pichinde Virus Infection in the Syrian Golden Hamster by Species-Specific Kinome Analysis\"Falcinelli et al., Mol. Cell. Proteomics. (2015) - PMID: 25573744 \"Histone H2A and H4 N-Terminal Tails are Positioned by the MEP50 WD-Repeat Protein for Efficient Methylation by the PRMT5 Arginine Methyltransferase\"Burgos et al., J. Biol. Chem. (2015) - PMID: 25713080

Application Notes for Enzyme Substrate Microarrays\"Exploring the human acetylome using high-density peptide microarrays\"Masch et al., Application Note (2012) (full text)\"Unraveling Phosphorylation of the Splicing Factor SC35 by the Dual Signaling Kinase PKC in Human T-Cells\" Rao et al., Application Note (2016) (full text)\"Use of High-Density Histone Peptide Arrays for Parsing the Specificity of a Histone Modifying Enzyme Complex\" Wilczek et al., Application Note (2013) (full text)

Testimonial \"We have successfully applied JPTs peptide microarrays as a screening tool for potential kinases transfering phosphate to phosphorylation sites identified by mass spectrometry. The triplicate alignment as well as the thorough selection of controls enabled generation of high quality data confirming our in vivo data generated by means of affinity chromatographic procedures.\" Marcelo P. Coba, PhD, The Wellcome Trust Sanger Institute, Cambridge, UK

Annotated Phosphosites-Kinase