Multiwell RepliTope™ BKV (large T antigen)

Product Code: RT-MW-0005

Peptide microarray displaying 170 peptides in 21 subarrays (3 copies / subarray) for incubation with 21 samples in parallel using an Array Slide 24-4 chamber. A peptide scan (15mers with 11 aa overlap) through Large T antigen (Swiss-Prot ID: P14999) of BK polyomavirus is displayed.Amount: 1 peptide microarray (each peptide is deposited 3x per subarray, 21 subarrays per slide)Purity: Free of truncated sequencesDelivery Format: Peptides immobilized onto glass slide (3 x 1 inches; 75 by 25 mm)Application(s): Immune monitoring, Humoral immune response, Seromarker discovery, Antibody epitope discovery, Binding experimentsIndication(s)/Topic(s): Infection, AIDS, Cancer chemotherapy, Transplantation, Merkel cell carcinoma, PML and BK nephropathyDelivery Time: 10-12 daysFor the incubation of Multiwell RepliTope™ Microarrays with different samples an incubation chamber such as Array slide chamber 24-4 is needed!

JPTs RepliTope™ Multiwell Peptide Microarrays JPT Peptide Technologies applies its unique peptide microarray platform to generate peptide microarrays on glass slides for biomarker discovery, immune monitoring, detection and validation of protein interactions. Overlapping peptides are immobilized on glass slides via a flexible linker by chemoselective coupling. The covalently attached peptides are displayed in three copies per subarray on each slide. The 21 subarrays can be incubated with different samples using an Array slide chamber 24-4 and regular ELISA equipment (washer, incubator). Read-out via fluorescence results in low background and high sensitivity.

Benefits of Multiwell RepliTope™ Peptide Microarrays - Incubate several different samples on one microarray slide- Get hundreds of identical microarray copies - Peptides are free of truncated sequences - Small amounts of your precious samples are needed for incubation - Applicable to a variety of biological samples (e.g. serum, plasma, antibody)- Low background on glass surface - Read-out via fluorescence

Multiwell RepliTope™ BKV (large T antigen)

Selected References for JPTs Peptide Microarrays\"Structure of GPN-loop GTPase Npa3 and Implications for RNA Polymerase II Assembly\" Niesser t al., Mol Cell Biol. (2015) - PMID: 26711263 \"Protective Efficacy of Adenovirus-protein Vaccines Against SIV Challenges in Rhesus Monkeys\" Barouch et al., Science (2015) - PMID: 26138104\"Identification and Characterization of Common B Cell Epitope in Bovine Leukemia Virus via High-throughput Peptide Screening System in Infected Cattle\" Bai et al., Retrovirology (2015) - PMID: 26715158\"High-Throughput Microarray Incubations Using Multi-Well Chambers\" Zerweck et al., Methods Mol Biol. (2015) - PMID: 26490464\"Peptide Microarrays for Coverage of Sequence Diversity in Monitoring the B cell Immune Response (TECH2P.911)\" Castro, et al., The Journal of Immunology (2015) - PMID: n.a.\"Antibodies to Influenza Nucleoprotein Cross-react with Human Hypocretin Receptor 2\" Ahmed et al., Science Translational Medicine (2015) - PMID: 26136476\"Serum Reactome Induced by Bordetella Pertussis Infection and Pertussis Vaccines: Qualitative Differences in Serum Antibody Recognition Patterns Revealed by Peptide Microarray Analysis\" Valentini et al., BMC Immunol. (2015) - PMID: 26129684

Application Notes\"A Modular Approach for Epitope Discovery and High-Resolution Profiling of Humoral Immune Responses\" Pawlowski et al. (2013) Full text\"Multiple Sclerosis and Epstein Barr Virus Infection. An Epitope Mapping Study\" Reimer et al. (2014) Full text\"The Challenge of Antigen Sequence Diversity:Solutions with ULTRA Peptide Libraries\" Pawlowski et al. (2015) Full text\"Rapid Mimotope Optimization for Pharmacokinetic Analysis of the Novel Therapeutic antibody IMAB362\" Daneschar et al. (2014) Full text

Testimonials for JPTs Peptide Microarrays \"Over the past five years my research team has been active in the development of species-specific peptide arrays. Our ability to advance this technology into new research areas has been dependent on working with the highest quality of arrays. In this regard, JPTs commitment to excellence has been a critical foundation for the success of our research program. I have been consistently impressed with their customer service, technical expertise and professionalism. When it comes to peptide work there are no other options for our group, JPT is the best.\"Scott Napper, PhD, Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Canada\"The RV 144 HIV trial is considered as one of first successful HIV vaccine trials. It has become clear that the V2 loop of gp120 is an important site for immunogenicity and protection from HIV infection. The use of JPTs PepStar™ microarray technology has been very useful for the correlation of the clincial outcome with humoral immune responses. As have the cyclic peptides been from JPT to validate these findings!\"Jeff Currier, PhD, Walter Reed Army Institute, Rockville, Maryland, USA \"We study modulation of dopaminergic neurotransmisson by TAAR1 and D2R. The functional interaction of the two receptors in the brain supports TAAR1 as a target for the treatment of psychiatric disorders such as schizophrenia or bipolar disorder. To map the epitopes of our newly generated specific anti-rat TAAR1 antibodies we used JPTs PepStar™ peptide microarrays. The peptide microarrays greatly contributed to our successful and recently published study. We were very satisified with the exceptional product and service delivered by JPT Peptide Technologies as well as their scientific Customer Support which was always at our disposal.\" Stefan Obermüller, F.Hoffmann La Roche Ltd., Roche Pharma Research and Early Development, Basel, Switzerland

Multiwell RepliTope™ BKV (large T antigen)