NBHistonePeptideH31:17K4me3(H3.11t233C)
ProductCode:SP-His_nb_044
SyntheticHistonepeptidecarrying1modification(K4me3)derivedfromhumanHistoneH3.11t233C(Swiss-ProtIDs:Q71DI3,P84243,P68431,Q6NXT2,Q16695).
Aminoacidsequence:H-ART-Lys(Me3)-QTARKSTGGKAPR-NH2
Amount:1vialcontaining30nmol(approx.100µg)
Purity:>70%(HPLC-MS)
Deliveryformat:Freeze-driedinambertypeglassvials
Application(s):Enzymekinetics,SubstrateSpecifity,Substrateidentification,Competitionexperiments,Co-crystallization,NMRstudies
Indication(s)/Topic(s):Cancer,Kinase,Methyltransferase,Acetyltransferase,Histones,Phosphatase,Generegulationandexpression,Epigenetics,Cellsignaling,Nucleosome
Deliverytime:10-12days
JPT"sNon-BiotinylatedHistoneCodePeptides
Inadditiontoourbiotinylatedhistonepeptides,JPToffersanon-biotinylatedlibraryofsyntheticposttranslationallymodifiedandunmodifiedhumanhistonepeptidesthatisnowavailableasindividualpeptides.Thepeptidelibraryconsistsof160peptidesfromdifferenthistonefamilies.PeptidescarryPTMsatreportedsites(e.g.acetylation,methylation,phosphorylation...)invariouscombinationsrepresentingnaturalvariants.
JPT"shighlyskilledandcommittedscientificstaffensuresthatthemostappropriatemethodsandtechniquesareselectedforeverysynthesisproject.AllofJPT"scatalogpeptidesareprovidedwithHPLC-MSanalysestoconfirmtheidentityanddemonstratethehighqualityofourpeptides.
BenefitsofJPT"sHistoneCodePeptides
-WholeproductionperformedinaregulatedEuropeanenvironmentaccordingtoDINENISO9001:2015&GCLPstandards;auditswelcome!
-Forhigh-throughputscreeningJPT"sHistoneCodePeptideMicroarrayisavailabledisplayingmorethan3000peptides.
-ProvisionoffreezedriedaliquotsforenhancedstABIlity
-Proventrackrecordformanyapplications
NBHistonePeptideH31:17K4me3(H3.11t233C)
SelectedReferencesforHistonePeptideTools
"HistoneH2AandH4N-TerminalTailsarePositionedbytheMEP50WD-RepeatProteinforEfficientMethylationbythePRMT5ArginineMethyltransferase"
Burgosetal.,J.Biol.Chem.(2015)-PMID:25713080
"H3K23me2isaNewHeterochromaticMarkinCaenorhaBDitiselegans"
Vandammeetal., NucleicAcidsRes.(2015)-PMID:26476455
"StructureoftheArginineMethyltransferasePRMT5-MEP50RevealsaMechanismforSubstrateSpecificity"
Hoetal.PLOSONE(2013)-PMID:23451136
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistone-ModifyingEnzymeComplex"
Shechteretal,ApplicationNote(2013)(fulltext)
"ComprehensivePeptideMicroarraysforHistoneResearch"
Schnatbaumetal,32thEPS(2012)(fulltext)
ApplicationNotes
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistone-ModifyingEnzymeComplex"
Shechteretal.ApplicationNote(2013)(fulltext)
"ComprehensiveCharacterizationofAntibodiesDirectedtowardsEpigeneticHistone-Modifications"
Maschetal.ApplicationNote(2015)(fulltext)
TestimonialforHistoneCodePeptideMicroarray
"JPT"snewextremelyhigh-densityhistonepeptidearrayisadramaticleapforwardforanalysisofthehistonecodehypothesis.Thecompletesequencecoverageacrosscorehistonesandhistonevariantsaswellasthepresenceofcommonandrarehistonepost-translationalmodifications "aloneandincombination"makesthisanunparalleledtoolforstudyingthe"writers"and"readers"ofthehistonecode.MylaboratoryhasusedthesearraystoaskthesequenceandPTMdependenceofahistonewriterenzymeandwehavediscoveredanactivityspecifyingcodethatwillkeepusbusyforyearstocome."
DavidShechter,PhD,DepartmentofBiochemistry,AlbertEinsteinCollegeofMedicine,YU(NY,USA)
NBHistonePeptideH31:17K4me3(H3.11t233C)
| HistoneFamily | HistoneH3 |
| Modification | Methylation |
| SequencePosition | Tail(1-42) |
| Isoform | H3.1 H3.1t H3.2 H3.3 H3.3C |
| Biotinylation | NoBiotin |
| Application | Substrateidentification |
| ProteinName | Histones |
| Organism | Homosapiens(Human) |
| Indication/Topic | Acetyltransferase Epigenetics Kinase |
| Numberofpeptides | 1 |
FurtherInformation:
| Amount | 1vialcontaining30nmol(approx.100µg) |
| Specifications | SyntheticHistonepeptidecarrying1modification |
| Documentation | ProductDatasheet |
| Swiss-ProtID | Q71DI3, P84243, P68431, Q6NXT2, Q16695 |
| NCBI | |
| Sequence | H-ART-Lys(Me3)-QTARKSTGGKAPR -NH2 |
